Analysis Note

ControlB95-8 cell lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Anti-EBNA2 Antibody, clone R3 is a Rat Monoclonal Antibody for detection of EBNA2 also known as Epstein-Barr nuclear antigen 2 & has been validated in WB, IP & IHC.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Gross, H., et al. (2010). Virology. 397(2):299-310.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kremmer, E., et al. (1995). Virology. 208(1):336-342.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gross, H., et al. (2010). Virology. 397(2): 299-310.PMID: target=′_blank', window.location);" rel="nofollow" href="http://www.ncbi.nlm.nih.gov/pubmed/19969318′ target=′_blank">19969318Kremmer, E., et al. (1995). Virology. 208(1): 336-342.PMID: target=′_blank', window.location);" rel="nofollow" href="http://www.ncbi.nlm.nih.gov/pubmed/11831716′ target=′_blank">11831716

The Epstein-Barr virus or EBV is associated with several human cancers and actively immortalizes B lymphocytes. EBV is leads to expression by a small group of viral proteins, including Epstein-Barr nuclear antigen 2 (EBNA2) which is required for immortalization. EBNA2 is a transcriptional activator belonging to the herpesviridae EBNA2 family, and performs a crucial role in activating the host resting B-cell and inducing B-cell proliferation. EBNA2 possesses an acidic activating domain with which it is able to recruit various cellular and viral involved in B-cell immortalization. Implicated in cellular phenotypic modifications, EBNA2 is known to upregulate the expression of cell surface receptors, such as CD21, MYC, and CD23, as well as upregulating viral genes such as LMP1, LMP2A, LMP2B, and EBNA1-6.

Immunogen

GST-tagged recombinant protein corresponding to human EBNA2.

Epitope: C-terminus of EBNA2

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Western Blot in B95-8 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected EBNA2 on 10 µg of B95-8 cell lysate.

Specificity

Demonstrated to react with human cell infected with EBV.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 75 kDa observed; Although the calculated molecular weight is 49 kDa, this protein is typically observed at ~ 75-80 kDa